High potency inhibitor of smooth muscle contractions
Applications in nephrology, stem cell studies, cardiovascular, cancer, and neurology
Highly cited
Y-27632 is a highly potent, cell permeable, selective and ATP competitive inhibitor of ROCK1 and ROCK2 (IC50=800nM). It acts as a potent inhibitor of agonist-induced Ca2+sensitization of myosin phosphorylation and smooth muscle contractions, blocking cell spreading and suppresses RhoA-induced formation of stress fibers in hepatic stellate cells. Y-27632 significantly reduces the increase of inflammatory cytokines after reperfusion, preventing the development of acute renal failure. There are numerous application for this compound including: cardioprotective effects, suppression of tumor cell invasion, inhibition of superoxide production, mimicking effect of β-agonists on human cells, and an antinociceptive effect.
There has been a growing interest in Y-27632 for use in stem cell self-renewal and reprogramming. It is known to increase the survival rate of human embryonic stem cells undergoing cryopreservation.
Product Details
| Alternative Name: | (R)-(+)-trans-N-(4-Pyridyl)-4-(1-aminoethyl)-cyclohexanecarboxamide . 2HCl |
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| Formula: | C14H21N3O . 2HCl |
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| MW: | 247.3 . 73.0 |
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| CAS: | 129830-38-2 |
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| Purity: | ≥98% (HPLC (UV)) |
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| Purity Detail: | Enantiomeric excess ≥96% |
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| Identity: | Determined by MS and NMR. |
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| Appearance: | White to pale yellow powder. |
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| Solubility: | Soluble in DMSO (25mg/ml), methanol (25mg/ml), acetonitrile (<1mg/ml), dioxan (<1mg/ml), water (very soluble). |
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| Shipping: | Ambient |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stock solutions are stable for up to 1 month when stored at -20°C. |
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| Handling: | Protect from light. Packaged under inert gas. After reconstitution, prepare aliquots and store at -20°C. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Biomechanical Strain Promotes the Differentiation of Murine Oogonial Stem Cells: J.A. MacDonald, et al.; Stem Cells Dev. (2021), Abstract;
Deciphering cell signaling networks with massively multiplexed biosensor barcoding: J-M. Yang, et al.; Cell 184, 6193 (2021), Abstract;
Deciphering cell signaling networks with massively multiplexed biosensor barcoding: J-M. Yang, et al.; Cell 184, 6193 (2021), Abstract;
Epigenetic modulation of immune synaptic-cytoskeletal networks potentiates γδ T cell-mediated cytotoxicity in lung cancer: R.R. Weng, et al.; Nat. Commun. 12, 2163 (2021), Abstract; Full Text
Epithelial miR-141 regulates IL-13-induced airway mucus production: S. Siddiqui, et al.; JCI Insight 5, 139019 (2021), Abstract;
Establishment of porcine and monkey colonic organoids for drug toxicity study: H. Li, et al.; Cell Regen. 10, 32 (2021), Abstract;
Identification and characterization of a novel Epstein-Barr Virus-encoded circular RNA from LMP-2 Gene: K. E. Tan, et al.; Sci. Rep. 11, 14392 (2021), Abstract;
LFA-1 and Kindlin-3 enable the collaborative transport of SLP-76 microclusters by myosin and dynein motors: K.P. Eidell, et al.; J. Cell Sci. 36, 258602 (2021), Abstract;
Long-Term Maintenance of Viable Adult Rat Sertoli Cells Able to Establish Testis Barrier Components and Function in Response to Androgens: H. Kabbesh, et al.; Cells 10, 2495 (2021), Abstract;
Mouse papillomavirus type 1 (MmuPV1) DNA is frequently integrated in benign tumors by microhomology-mediated end-joining: L. Yu, et al.; PLoS Pathog. 17, e1009812 (2021), Abstract;
Probing TGF-β1-induced cytoskeletal rearrangement by fluorescent-labeled silica nanoparticle uptake assay: H.R. Shin, et al.; Biochem. Biophys. Rep. 28, 101137 (2021), Abstract;
Proteomic Analysis of Exosomes during Cardiogenic Differentiation of Human Pluripotent Stem Cells: P. Ashok & E.S. Tzanakakis; Cells 10, 2622 (2021), Abstract;
Proteomic Analysis of Exosomes during Cardiogenic Differentiation of Human Pluripotent Stem Cells: P. Ashok & E.S. Tzanakakis; Cells 10, 2622 (2021), Abstract;
Reprogramming of two induced pluripotent stem cell lines from a heterozygous GRIN2D developmental and epileptic encephalopathy (DEE) patient (BGUi011-A) and from a healthy family relative (BGUi012-A): T. Rabinski, et al.; Stem Cell Res. 51, 102178 (2021), Abstract;
Reuse of Cell Culture Inserts for in vitro Human Primary Airway Epithelial Cell Studies: T. Kato, et al.; Am. J. Respir. Cell Mol. Biol. (2021), Abstract;
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