最近的研究表明，維生素D水平影響各種疾病狀態，并與人類健康的許多指標相關聯。這些疾病包括骨質疏松癥和關節炎等骨骼疾病，也包括高血壓、糖尿病、癌癥和心臟病等其他疾病。Enzo Life Sciences的25-羥基維生素D ELISA試劑盒為快速便捷檢測人血漿和血清中25-羥基維生素D水平提供了一種方法。
維生素D向活性形式的轉化始于7-脫氫膽固醇被紫外線作用形成母體維生素D3。或者來源于食物攝取的母體維生素D2。這些母體化合物被運輸到肝臟并羥基化為25(OH)維生素D。這種代謝物隨后被運輸到腎臟，再次羥基化為1，25(OH)2維生素D，這是維生素D2的生物活性形式。維生素D代謝物的水平隨著母體維生素D攝取量的增加而成比例增加。與活性形式相比，25(OH)維生素D在循環中的半衰期和穩定性更長，這是25(OH)維生素D代謝物的檢測被用作總維生素D濃度指標的原因。
產品信息
產品貨號 | ADI-900-215-0001 |
產品名稱 | 25(OH) Vitamin D ELISA kit/欣博盛生物 |
規格 | 96 wells |
靈敏度 | 1.98 ng/ml |
檢測范圍 | 0.5-1010 ng/ml |
其他名稱 | 25-Hydroxyvitamin D |
檢測時間 | ~1.5 hours |
適用樣本類型 | 血清，血漿 |
適用種屬 | 人 |
交叉反應 | 25(OH) Vitamin D3 (100%), 25(OH) Vitamin D2 (~80%), 1,25(OH)2-Vit. D2 (>100%), 1,25(OH)2-Vitamin D3 (>100%), 3-epi 25(OH) Vitamin D3 (~10%), 24,25(OH)2-Vitamin D3 (~6%) and<1%: 1α(OH) Vitamin D2, 1α(OH) Vitamin D3, Vitamin D2, Vitamin D3 |
試劑盒組分 | Microtiter Plate, Conjugate Concentrate, Antibody, Dissociation Buffer, Conjugate Diluent, Sample Diluent, Wash Buffer Concentrate, Standards, Substrate, Stop Solution, Assay Layout |
標準曲線示例
部分產品文獻引用
1. Tri-ortho-cresyl phosphate exposure leads to low egg production and poor eggshell quality via disrupting follicular development and shell gland function in laying hens: J. Li, et al.; Ecotoxicol. Environ. Saf. 225, 112771 (2021)
2. Vitamin K and D Status in Patients with Knee Osteoarthritis: An Analytical Cross-sectional Study: A. Askari, et al.; Mediterr. J. Rheumatol. 32, 350 (2021)
3. Compounding effect of vitamin D3 diet, supplementation, and alcohol exposure on macrophage response to mycobacterium infection: M.E. Gough, et al.; Tuberculosis (Edinb.) 116S, S42 (2019), Application(s): ELISA using culture supernatants
4. VDR gene methylation as a molecular adaption to light exposure: Historic, recent and genetic influences: E.L. Beckett, et al.; Am. J. Hum. Biol. 29, (2017)
5. A study of Vitamin D Levels in hypothyroid patients, a case control study in a tertiary care hospital of central India: R. Kohokare, et al.; NJMDR 4, 89 (2016), Application(s): Vitamin D levels were measured from serum of hypothyroid patients
6. Evaluation of vitamin D relationship with type 2 diabetes and systolic blood pressure: S.B. Nayak, et al.; BMJ Open Diabetes Res. Care 4, e000285 (2016), Application(s): Determination of 25(OH)D levels in human plasma and whole blood samples
7. Reduced 25-OH vitamin D in patients with autoimmune cytopenias, clinical correlations and literature review: B. Fattizzo, et al.; Autoimmun. Rev. 15, 770 (2016), Application(s): Measured Vitamin D in 40 year old humans
8. Relationship between methylation status of vitamin D-related genes, vitamin D levels, and methyl-donor biochemistry: E.L. Beckett, et al.; J. Nutr. Int. Metab. 6, 8 (2016), Application(s): ELISA using human plasma,
9. Vitamin D deficiency is associated with endothelial dysfunction and increases type I interferon gene expression in a murine model of systemic lupus erythematosus: J.A. Reynolds, et al.; Arthritis Rheumatol. 68, 2929 (2016)
10. Vitamin D status in different stages of disease severity in dogs with chronic valvular heart disease: T. Osuga, et al.; J. Vet. Intern. Med. 29, 1518 (2015), Application(s): ELISA using dog serum
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