CUTANA pAG-Tn5是靶向剪切及轉座酶(CUT&Tag)技術中進行高效繪制染色質特征的關鍵試劑。與ChIP-seq相比，CUT&Tag在降低細胞需求量和測序深度的信噪比方面進行了顯著改進。CUTANA pAG-Tn5是一種高活性的E. coli轉座酶突變體(Tn5)與蛋白A/G的融合產物，可與來自多種物種宿主的靶抗體兼容。它也沒有表位標簽，使其適合于標簽介導的CUT&Tag(如FLAG、HA、TY1、V5等)。該產品經過高度純化，已去除污染的E. coli DNA，并在細胞數量少的情況下進行復雜分析。為了每次實驗的標準化以及抗體驗證和反應監測，推薦使用SNAP-CUTANA™ nucleosome spike-ins(如EpiCypher 19-1002)。Tn5帶有mosaic 接頭，可在CUT&Tag中使用。
Adapters
Tn5ME-A: 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3’
Tn5ME-B: 5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3’
Tn5ME-rev: 5’-[phos]CTGTCTCTTATACACATCT-3’
組分
50 mM HEPES-KOH pH 7.2, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% glycerol.
保存條件
Stable for one year at -20℃ from date of receipt. The protein is not subject to freeze/thaw under these conditions.
數據示例
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FIGURE 1: Protein gel data. CUTANA pAG-Tn5 for CUT&Tag (1 µg) was resolved via SDS-PAGE and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated. Uncharged pAG-Tn5 monomer is 78.5 kDa, however once charged with DNA, Tn5 dimerizes to a final complex weight of 191 kDa. |
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FIGURE 2: Size distribution of released chromatin. CUT&Tag was performed as described above. Recovered DNA was directly PCR amplified to produce sequenceready libraries. Agilent TapeStation traces for libraries derived from negative control IgG (top) and H3K27me3 (bottom) antibodies are shown. Excised DNA is highly enriched for mononucleosomes (peak at ~300 bp reflects ~150 bp insert size). |
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FIGURE 3: CUT&Tag data. CUT&Tag was performed as described above. Representative sequencing tracks obtained using CUTANA pAG-Tn5 show a 229 kb close up view of the LAMC3 gene. CUTANA pAG-Tn5 produced clear peaks with genomic distribution profiles consistent with the known biological functions of H3K4me3 and H3K27me3 as well as minimal background in the IgG negative control. |
訂購詳情
貨號 | 產品名稱 | 規格 |
15-1017 | CUTANA™ pAG-Tn5 for CUT&Tag | 50 Reactions |
15-1117 | CUTANA™ pAG-Tn5 for CUT&Tag | 250 Reactions |
如需了解更多詳細信息或相關產品，請聯系EpiCypher中國授權代理商-欣博盛生物
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