Enzo Life Sciences公司的NUCLEAR-ID® Blue DNA stain (GFP-CERTIFIED®)和NUCLEAR-ID ® Red DNA Stain是細胞滲透性染料，適用于活細胞的細胞核染色。這系列染料為通過流式細胞術研究細胞周期進程的誘導和抑制提供了一種簡便方法。該試劑在活細胞研究中的潛在應用包括確定細胞DNA含量和細胞周期分布、檢測生長模式的變化、監測細胞凋亡以及評估腫瘤細胞行為和抑制基因機制。
NUCLEAR-ID® Red DNA stain
產品特點
● 遠紅色熒光特異性DNA染料
● 穩定且純度高
● 可分析活細胞、可滲透細胞和固定細胞
● 無光漂白作用
● 無需RNase處理
● 與GFP和FITC兼容
● 不需要紫外線激光源激發
● 經驗證適用于各種細胞密度
● 快速且易于使用
實例分析
在37°C下用終濃度為4000x或2000x的NUCLEAR-ID ® Red DNA Stain或同等µM濃度的競爭品牌染料對細胞進行染色，并在染色后輕輕洗滌。在15分鐘和24小時對細胞成像，結果顯示NUCLEAR-ID ® Red DNA Stain在更低的濃度就能顯現dsDNA。
產品詳情：
產品名稱 | NUCLEAR-ID® Red DNA stain |
產品貨號 | ENZ-52406 |
產品規格 | 200µl |
最大激發波長 | 566nm |
最大發射波長 | 650nm |
純度 | ≥93%（HPLC） |
質量控制 | 1.Absorption peak of NUCLEAR-ID® Red dye: λmax = 566 ± 4 nm 2.% purity of NUCLEAR-ID® Red dye by HPLC: ≥93% |
應用 | Flow Cytometry, Fluorescent detection |
短期儲存 | -20°C |
長期儲存 | -80°C |
產品說明 | The NUCLEAR-ID® Red DNA Stain is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
部分產品文獻引用
1. CD200R1L is a functional evolutionary conserved activating receptor in human neutrophils: M.I.P. Ramos, et al.; J. Leukoc. Biol. 111, 367 (2022)
2. FOXM1 network in association with TREM1 suppression regulates NET formation in diabetic foot ulcers: A.P. Sawaya, et al.; EMBO Rep. 23, e54558 (2022)
3. Multi-dimensional-double-spiral (MDDS) inertial microfluidic platform for sperm isolation directly from the raw semen sample: H. Jeon, et al.; Sci. Rep. 12, 4212 (2022)
4. DYRK1A negatively regulates CDK5-SOX2 pathway and self-renewal of glioblastoma stem cells: B. Chen, et al.; Int. J. Mol. Sci. 22, 4011 (2021)
5. Global phosphoproteomics reveals DYRK1A regulates CDK1 activity in glioblastoma cells: A. Recasens, et al.; Cell Death Discov. 7, 81 (2021)
6. Hydrogen Peroxide Is Crucial for NLRP3 Inflammasome-Mediated IL-1β Production and Cell Death in Pneumococcal Infections of Bronchial Epithelial Cells; Journal of Innate Immunity: S. Surabhi, et al.; J. Innate Immun. 10, 1159 (2021)
7. Licochalcone A inhibits hypoxia-inducible factor-1α accumulation by suppressing mitochondrial respiration in hypoxic cancer cells: M.K. Park, et al.; Biomed. Pharmacother. 133, 111082 (2021)
NUCLEAR-ID® Blue DNA stain (GFP-CERTIFIED®)
產品特點
● 藍色熒光特異性DNA染料
● 穩定且純度高
● 可分析活細胞、可滲透細胞和固定細胞
● 無光漂白作用
● 無需RNase處理
● 與GFP和紅色染料兼容
● 經驗證適用于各種細胞密度
● 快速易用
產品詳情：
產品名稱 | NUCLEAR-ID® Blue DNA stain (GFP-CERTIFIED®) |
產品貨號 | ENZ-CHM103-0200 |
產品規格 | 200µl |
最大激發波長 | 350nm |
最大發射波長 | 461nm |
純度 | ≥95% (HPLC) |
外觀 | Frozen liquid |
應用 | Flow Cytometry, Fluorescent detection |
短期儲存 | -20°C, Protect from light. Avoid freeze/thaw cycles |
長期儲存 | -80°C, Protect from light. Avoid freeze/thaw cycles |
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